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Fast atom bombardment
Fast atom bombardment (FAB) is an ionization technique used in mass spectrometry in which a beam of high energy atoms strikes a surface to create ions. It was developed by Michael Barber at the University of Manchester.〔Barber, M.; Bordoli, R.S.; Sedgewick, R.D.; Tyler, A.N., Nature, 293, 1981, pp270-275〕 When a beam of high energy ions is used instead of atoms, the method is known as liquid secondary ion mass spectrometry.〔
〕 The material to be analyzed is mixed with a non-volatile chemical protection environment called a matrix and is bombarded under vacuum with a high energy (4000 to 10,000 electron volts) beam of atoms. The atoms are typically from an inert gas such as argon or xenon. Common matrices include glycerol, thioglycerol, 3-nitrobenzyl alcohol (3-NBA), 18-crown-6 ether, 2-nitrophenyloctyl ether, sulfolane, diethanolamine, and triethanolamine. This technique is similar to secondary ion mass spectrometry and plasma desorption mass spectrometry.
==How it works==
FAB is a relatively low fragmentation (soft) ionization technique and produces primarily intact protonated molecules denoted as (+ H )+ and deprotonated molecules such as (- H )−. The nature of its ionization mechanism is similar to electrospray ionization and matrix-assisted laser desorption/ionization (MALDI).
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